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Research Fellow/Lu, Mei-Kuang

Lu, Mei-Kuang
Current Position:Research Fellow
Tel:2820-1999 ext. 7391、7531

Ph.D., School of Biological Sciences, University of Nebraska-Lincoln, USA
Major Professional Experiences
Associate Research Fellow, National Research Institute of Chinese Medicine
Plant Tissue Culture, Fungal Culture, Carbohydrate Chemistry
Research Description
I have been interested in polysaccharides production, structure and biological function in fungi. Much of the work I have done in the past used Antrodia cinnamomea as a model for polysaccharide structure and function. For the past three years Dr. Jing-Jy Cheng from this Institute and myself have been working on production, purification, and characterization of polysaccharides in fungi and how this polysaccharides responds to vascular endothelial growth factor (VEGF)-induced tube formation in endothelial cells (ECs). My interests in this system is how to use cultured mycelia to provide the opportunity to fine-tune the biosynthetic pathway of polysaccharide. These polysaccharides from different sources could differ somewhat in structure and composition. Different physicochemical parameters of polysaccharides, such as solubility, primary structure, molecular weight, extent of branching by side-chain substituents, and the charge on the polymer, all appear to influence their biological activities. In preparation the bioactive sulfated polysaccharide (SPS) of Antrodia cinnamomea we discovered that a sulfated salt (SO42-) could change in the sugar compositions, and degree of sulfation in the SPS. The more the SO42-, the higher the sulfation degree. We observed that the degree of sulfation affected the antiangiogenic and neuroprotective properties. These results are consistent with those of Parish et al. who found that the inhibitory activity against human angiogenesis of SPSs was critically dependent on the chain length and degree of sulfation. We are continuing to characterize the minimum length of sulfated oligosaccharides with the greatest anti-angiogenic activity by using fragmentation of SPS. We also have Dr. Chia-Chuan Chang in this Institute to join to elucidate partial structural of fungal polysaccharide by using 1H, 13C and 2D NMR spectroscopy, including NOESY and HMBC experiments for linkage and sequence analysis. Recently, we discovered a novel 1,6-branched 1,3-α-D-galactan from cultured mycelia of Poria cocos.
Published Papers
NCBI PubMed :  Mei-Kuang Lu (Please click!!)
  1. Mei-Kuang Lu #, *, Chia-Chuan Chang #, Chi-Hsein Chao, Yu-Chi Hsu (2022) Structural changes, and anti-inflammatory, anti-cancer potential of polysaccharides from multiple processing of Rehmannia glutinosa. International Journal of Biological Macromolecules, in press. # Equally contribute
  2. Meng-Hsin Lee, Wei-Bin Lu, Mei-Kuang Lu *, Fi-John Chang * (2022) A hybrid of response surface methodology and artificial neural network in optimization of culture conditions of mycelia growth of Antrodia cinnamomea. Biomass and Bioenergy 158, 106349 . 
  3. Mei-Kuang Lu*, Chi-Hsein Chao, Yu-Chi Hsu (2021) Effect of carbohydrate-feeding strategy on the production, physiochemical changes, anti-inflammation activities of polysaccharides of Poria cocos. International Journal of Biological Macromolecules 192, 435-443.
  4. Mei-Kuang Lu, Chi-Hsein Chao, Yu-Chi Hsu, Chia-Chuan Chang * (2021) Structural sequencing and anti-inflammatory, anti-lung cancer activities of 1,4-α/β-sulfomalonoglucan in Antrodia cinnamomeaInternational Journal of Biological Macromolecules 170, 307-316.
  5. Tung-Yi Lin # *, Mei-Kuang Lu #, Ai-Jung Tseng, Chi-Hsein Chao (2020) Effects of sterol-type elicitors on biochemical characterization of polysaccharides from Antrodia cinnamomea. International Journal of Biological Macromolecules 162, 1476-1483. # Equally contribute
  6.  Meng-Hsin Lee, Chi-Hsein Chao, Yu-Chi Hsu, Mei-Kuang Lu * (2020) Production, characterization, and functions of sulfated polysaccharides from zinc sulfate enriched cultivation of Antrodia cinnamomeaInternational Journal of Biological Macromolecules 159, 1013-1021.
  7. Tung-Yi Lin §, Mei-Kuang Lu §, and Chia-Chuan Chang * (2020) Structural identification of a fucose-containing 1,3-β-mannoglucan from Poria cocos and its anti-lung cancer CL1-5 cells migration via inhibition of TGFβR-mediated signaling. International Journal of Biological Macromolecules 157, 311-318.  § Equally contribute
  8.  Mei-Kuang Lu *, Meng-Hsin Lee , Chi-Hsein Chao, Yu-Chi Hsu (2020) Physiochemical changes and mechanisms of anti-inflammation effect of sulfated polysaccharides from ammonium sulfate feeding of Antrodia cinnamomea. International Journal of Biological Macromolecules 148, 715-721. 
  9. Chun-Han Su, Mei-Kuang Lu, Ting-Jang Lu, Ming-Nan Lai, Lean-Teik Ng (2020) A (1→6)-branched (1→4)-β-D-glucan from Grifola frondosa inhibits lipopolysaccharide-induced cytokine production in RAW264.7 macrophages by binding to TLR2 rather than Dectin-1 or CR3 receptors. Journal of Natural Products 83, 231-242. 
  10. Tung-Yi Lin*, Chia-Chuan Chang, Ai-Jung Tseng, Chi-Hsein Chao, Mei-Kuang Lu* (2019) Sodium thiosulfate enhances production of polysaccharides and anticancer activities of sulfated polysaccharides in Antrodia cinnamomea. Carbohydrate Polymers 216, 204-212. 
  11. Tung-Yi Lin*, Ai-Jung Tseng, Wei-Lun Qiu, Chi-Hsein Chao, Mei-Kuang Lu * (2019) A sulfated glucan from Antrodia cinnamomea reduces Slug expression through regulation of TGFβ/AKT/GSK3β axis in lung cancer. Carbohydrate Polymers 210, 175-184.
  12. Mei-Kuang Lu *, Tung-Yi Lin, Chia-Chuan Chang * (2018) Chemical identification of a sulfated glucan from Antrodia cinnamomea and its anti-cancer functions via inhibition of EGFR and mTOR activity. Carbohydrate Polymers 202, 536-544.
  13. Tung-Yi Lin*, Ai-Jung Tseng, Chi-Hsein Chao, Mei-Kuang Lu* (2018) Microelements induce changes in characterization of sulfated polysaccharides from Antrodia cinnamomea. International Journal of Biological Macromolecules 120, 952-958. 
  14. Hsien-Yeh Hsu*, Tung-Yi Lin, Chun-Hao Hu, David Ta Fu Shu, Mei-Kuang Lu* (2018) Fucoidan upregulates TLR4/CHOP-mediated Caspase-3 and PARP activation to enhance cisplatin-induced cytotoxicity in human lung cancer cells. Cancer Letters 432, 112-120.
  15. Jing-Jy Cheng, Chi-Hsein Chao, Mei-Kuang Lu * (2018) Large-scale preparation of sulfated polysaccharides with anti-angionenic and anti-inflammatory properties from Antrodia cinnamomiaInternational Journal of Biological Macromolecules 113,1198-1205.
  16. Chia-Chuan Chang *, Jing-Jy Cheng, I-Jung Lee, Mei-Kuang Lu* (2018) Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea. International Journal of Biological Macromolecules 114,584-591
  17. Mei-Kuang Lu *,1, Tung-Yi Lin 1, Chun-Hao Hu, Chi-Hsein Chao,  Chia-Chuan Chang *, and Hsien-Yeh Hsu * (2017) Characterization of a sulfated galactoglucan from Antrodia cinnamomea and its anticancer mechanism via TGFβ/FAK/Slug axis suppression. Carbohydrate Polymers 167, 229-239.  Equally contribute
  18. Mei-Kuang Lu *, Tung-Yi Lin, Chi-Hsein Chao, Chun-Hao Hu, and Hsien-Yeh Hsu * (2017) Molecular mechanism of Antrodia cinnamomea sulfated polysaccharide on the suppression of lung cancer cell growth and migration via induction of transforming growth factor β receptor degradation. International Journal of Biological Macromolecules 95,1144-1155.
  19. Yun-Ting Chen, Yuh-Chiang Shen, Meng-Chi Chang, Mei-Kuang Lu * (2016) Precursor-feeding strategy on the triterpenoid production and anti-inflammatory activity of Antrodia cinnamomea. Process Biochemistry 51, 941-949.
  20. Feng-Lin Hsu, Hsiao-Chuan Chen, and Mei-Kuang Lu * (2016) Polysaccharides from Cunninghamia konishii promote the mycelial growth of Antrodia cinnamomea. Food Hydrocolloids 53, 2-6
  21. Jing-Jy Cheng, Chi-Hsein Chao, Pin-Chun Chang, Mei-Kuang Lu * (2016) Studies on anti-inflammatory activity of sulfated polysaccharides from cultivated fungi Antrodia cinnamomea. Food Hydrocolloids 53, 37-45
  22. Chang, C.-W., Lur, H.-S., Lu, M.-K., & Cheng, J.-J. (2013). Sulfated polysaccharides of Armillariella mellea and their anti-inflammatory activities via NF-κB suppression. Food Research International, 54(1), 239-245. doi:10.1016/j.foodres.2013.07.005
  23. Lee, M.-H., Chao, C.-H., & Lu, M.-K. (2012). Effect of carbohydrate-based media on the biomass; polysaccharides molecular weight distribution and sugar composition from Pycnoporus sanguineus. Biomass and Bioenergy, 47, 37-43. doi:10.1016/j.biombioe.2012.10.007
  24. Cheng, J.-J., Chang, C.-C., Chao, C.-H., & Lu, M.-K. (2012). Characterization of fungal sulfated polysaccharides and their synergistic anticancer effects with doxorubicin. Carbohydrate Polymers, 90(1), 134-139. doi:10.1016/j.carbpol.2012.05.005
  25. Kuo, C.-I., Chao, C.-H., & Lu, M.-K. (2012). Effects of auxins on the production of steroidal alkaloids in rapidly proliferating tissue and cell cultures of Solanum lyratum. Phytochemical Analysis, 23(4), 400-404. doi:10.1002/pca.1371
  26. Chao, C.-H., & Lu, M.-K. (2012). Effects of Carbon-Source on the Structural Changes of Polysaccharides in the Dietary Mushroom Agaricus subrufescens. Journal of Chinese Medicine, 23(1), 31-40.
  27. Chao, C.-H., Wu, H.-J., & Lu, M.-K. (2011). Promotion of fungal growth and underlying physiochemical changes of polysaccharides in Rigidoporus ulmarius, an edible Basidiomycete mushroom. Carbohydrate Polymers, 85(3), 609-614.  doi:10.1016/j.carbpol.2011.03.023
  28. Chang, T.-T., Chao, C.-H., & Lu, M.-K. (2011). Enhanced biomass production of Pycnoporus sanguineus and alterations in the physiochemical properties of its polysaccharides. Carbohydrate Polymers, 83(2), 796-801. doi:10.1016/j.carbpol.2010.08.055
  29. Cheng, J.-J., Lu, M.-K., Lin, C.-Y., & Chang, C.-C. (2011). Characterization and functional elucidation of a fucosylated 1,6-α-D-mannogalactan polysaccharide from Antrodia cinnamomea. Carbohydrate Polymers, 83(2), 545-553. doi:10.1016/j.carbpol.2010.08.016
  30. Men-Chi Chang, Wei-Fu Chien, Chi-Hsein Chao, Mei-Kuang Lu * (2010) Effects of cold stress on alterations of physiochemical and chemical properties of rice polysaccharides. Carbohydrate Polymers 80, 374-377.
  31. Mei-Kuang Lu1, Jing-Jy Cheng1, Cha-Yui Lin, Chia-Chuan Chang * (2010) Purification, structural elucidation, and anti-inflammatory effect of a water-soluble 1,6-branched 1,3-α-d-galactan from cultured mycelia of Poria cocos. Food Chemistry 118, 349-356. 1 Equally contribute.
  32. Jing-Jy Cheng 1, Huu-Sheng Lur1, Nai-Kuei Huang, Hsuan-Pei Chen, Cha-Yui Lin, Mei-Kuang Lu * (2009) Exploring the potential of biopharmaceutical production by Rigidoporus ulmarius: cultivation, chemistry, and bioactivity studies. Process Biochemistry 44, 1237-1244.
  33. Jing-Jy Cheng, Nai-Kuei Huang, Huu-Sheng Lur, Chung-Io Kuo, Mei-Kuang Lu * (2009) Characterization and biological functions of sulfated polysaccharides from sulfated-salt treatment of Antrodia cinnamomea. Process Biochemistry 44, 453-459.
  34. Jing-Jy Cheng, Cha-Yui Lin, Huu-Sheng Lur, Hsuan-Pei Chen, Mei-Kuang Lu * (2008) Properties and biological functions of polysaccharides and ethanolic extracts isolated from medicinal fungus, Fomitopsis pinicola. Process Biochemistry 43, 829-834.
  35. Mei-Kuang Lu, Jing-Jy Cheng, Wen-Lin Lai, Yen-Ru Lin, Nai-Kuei Huang (2008) Fermented Antrodia cinnamomea extract prevents rat PC12 cells from serum deprivation-induced apoptosis: the role of the MAPK family. Journal of Agricultural Food Chemistry 56, 865-874.
  36. Meng-Hsin Lee, Jing-Jy Cheng, Cha-Yui Lin, Yi-Jyun Chen, Mei-Kuang Lu * (2007) Precursor feeding strategy for the production of solanine, solanidine and solasodine by a cell culture of Solanum lyratum. Process Biochemistry 42, 899-903.
  37. Feng-Lin Hsu, Cheng-Jen Chou, Yi-Chieh Chang, Tun-Tschu Chang, Mei-Kuang Lu * (2006) Promotion of hyphal growth and underlying chemical changes in Antrodia cinnamomea by host factors from Cinnamomum cinnamomea. International Journal of Food Microbiology 106, 32-38.
  38. Mei-Kuang Lu, Jing-Jy Cheng, Wen-Lin Lai, Yen-Ru, Lin, and Nai-Kuei Huang (2006) Adenosine as an active component of Antrodia cinnamomea that prevents rat PC12 cells from serum deprivation-induced apoptosis through the activation of Adenosine A2A receptors. Life Sciences 79, 252-258.
  39. Shih Chung Chen1, Mei-Kuang Lu1, Jing-Jy Cheng , Danny Ling Wang (2005) Antiangiogenic activities of polysaccharides isolated from medicinal fungi. FEMS Microbiology Letters 249, 247-254. 1Equally contribute.
  40. Jing-Jy Cheng, Chao-Jan Yang, Chia-Hua Cheng, Ya-Ting Wang, Nai-Kuei Huang, Mei-Kuang Lu * (2005) Characterization and functional study of Antrodia camphorata lipopolysaccharide. Journal of Agricultural Food Chemistry 56, 865-874.
  41. Jing-Jy Cheng, Nai-Kuei Huang, Tun-Tschu Chang, Danny Ling Wang, Mei-Kuang Lu * (2005) Study for anti-angiogenic activities of polysaccharides isolated from Antrodia cinnamomea in endothelial cells. Life Sciences 76, 3029-3042.
  42. Nai-Kuei Huang, Jing-Jy Cheng, Wen-Lin Lai, Mei-Kuang Lu * (2005) Antrodia camphorata prevents rat pheochromocytoma cells from serum deprivation-induced apoptosis. FEMS Microbiol.ogy Letters 244, 213-219.
  43. Yuh-Chiang Shen, Cheng-Jen Chou, Yea-Hwey Wang, Chieh-Fu Chen, Yueh-Chinh Chou, Mei-Kuang Lu * (2004) Anti-inflammatory activity of the extracts from mycelia of Antrodia camphorata cultured with water-soluble fraction from five different Cinnamomum genera. FEMS Microbiology Letters 231, 137-143.
  44. Guei-Jane Wang , Hsiang-Wen Tseng , Cheng-Jen Chou , Tung-Hu Tsai , Chi-Tin Chen , Mei-Kuang Lu * (2003) The vasorelaxation of Antrodia camphorata mycelia: involvement of endothelial Ca2+-NO-cGMP pathway. Life Sciences 73, 2769-2783.
  45. I-Hung Lee, Ray-Ling Huang, Chi-Ting Chen,Hsiao-Chuan Chen, Wen-Chi Hsu, Mei-Kuang Lu * (2002) Antrodia camphorata polysaccharide exhibits anti-hepatitis B virus effects. FEMS Microbiology Letters 209, 63-67.
*Corresponding author

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